Endocrine Abstracts

Immunoassays for testosterone produce sometimes incorrectly high results in female samples. The reasons of this phenomenon are not fully understood, but interference by cross-reacting substances and inaccurate calibration can be critical. One known endogenous interfering substance is dehydroepiandrosterone sulphate (DHEA-S). Other substances still have to be identified.An Elecsys® Testosterone II assay using a new high affinity sheep mono...

Hyperthyroidism in Graves’ disease is due to TSH receptor (TSHR) autoantibodies (TRAb) directed against the TSH binding pocket. Detection of TRAb in clinical rou-tine is performed by 2nd gen assays using human or porcine TSHR. Such TSH bin- ding inhibition immunoglobulin (TBII) assays determine the ability of TRAb to inhi- bit the binding of labelled TSH to immobilized TSHR. TRAb are present in low con- centrations and mostly recognize conformational epitopes. The conform...